deltavision rt wide-field epifluorescence deconvolution microscope Search Results


deltavision microscope system  (Cytiva Europe)
92
Cytiva Europe deltavision microscope system
Deltavision Microscope System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/DeltaVision+OMX+Flex/pmc05706992-199-8-13
Average 92 stars, based on 1 article reviews
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emccds (evolve)  (Photometrics Inc)
90
Photometrics Inc emccds (evolve)
AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on <t>a</t> <t>DeltaVision</t> OMX Blaze system equipped with three <t>emCCDs.</t> Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.
Emccds (Evolve), supplied by Photometrics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/emccds++evolve+/pmc04442395-328-19-21
Average 90 stars, based on 1 article reviews
emccds (evolve) - by Bioz Stars, 2026-10
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orca ag camera  (Hamamatsu)
90
Hamamatsu orca ag camera
AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on <t>a</t> <t>DeltaVision</t> OMX Blaze system equipped with three <t>emCCDs.</t> Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.
Orca Ag Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/orca+ag+camera/pm17636000-232-26-25
Average 90 stars, based on 1 article reviews
orca ag camera - by Bioz Stars, 2026-10
90/100 stars
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deltavision  (Nikon)
99
Nikon deltavision
AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on <t>a</t> <t>DeltaVision</t> OMX Blaze system equipped with three <t>emCCDs.</t> Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.
Deltavision, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/Objectives/pmc07265327-364-16-25
Average 99 stars, based on 1 article reviews
deltavision - by Bioz Stars, 2026-10
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electron-multiplying charge-coupled devices (emccds; evolve)  (Photometrics Inc)
90
Photometrics Inc electron-multiplying charge-coupled devices (emccds; evolve)
AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on <t>a</t> <t>DeltaVision</t> OMX Blaze system equipped with three <t>emCCDs.</t> Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.
Electron Multiplying Charge Coupled Devices (Emccds; Evolve), supplied by Photometrics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/high+speed+electron+multiplying+charge+coupled+device++emccd++camera+evolve+delta/pmc04442395-146-18-23
Average 90 stars, based on 1 article reviews
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alexa fluor 488 goat anti mouse igg h l secondary antibody  (Jackson Immuno)
96
Jackson Immuno alexa fluor 488 goat anti mouse igg h l secondary antibody
AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on <t>a</t> <t>DeltaVision</t> OMX Blaze system equipped with three <t>emCCDs.</t> Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.
Alexa Fluor 488 Goat Anti Mouse Igg H L Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deltavision+rt+wide-field+epifluorescence+deconvolution+microscope/Alexa+Fluor+488+AffiniPure+Goat+Anti-Mouse+IgG/pm34826343-311-62-71
Average 96 stars, based on 1 article reviews
alexa fluor 488 goat anti mouse igg h l secondary antibody - by Bioz Stars, 2026-10
96/100 stars
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Image Search Results


AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on a DeltaVision OMX Blaze system equipped with three emCCDs. Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.

Journal: Journal of Virology

Article Title: AP-2-Associated Protein Kinase 1 and Cyclin G-Associated Kinase Regulate Hepatitis C Virus Entry and Are Potential Drug Targets

doi: 10.1128/JVI.02705-14

Figure Lengend Snippet: AAK1 and GAK regulate HCV entry at a postbinding step. (A) Cells transfected with siRNAs targeting AAK1, GAK, and SDC1 or an NT sequence as well as NT cells pretreated with heparinase I were infected with HCVcc at 4°C for 2 h. This was followed by removal of unbound virus, RNA extraction, and quantification of cell-bound HCV RNA by qRT-PCR as a measurement of HCV attachment. Shown are SDC1 protein levels in the indicated cells and HCV RNA levels relative to the NT control level. (B) Purification of highly infectious Dil-HCV particles. Concentrated HCV was labeled with Dil, layered on a 10 to 80% sucrose gradient, and purified by ultracentrifugation. The top of the gradient is fraction 1. Infectious HCV (solid line, left y axis) was quantified by focus formation (FF) assays, and HCV RNA copy numbers (dashed line, right y axis) were determined by RT-PCR. Dil-HCV was isolated from gradient fraction 3, which harbored the peak of infectivity and had a density of 1.06g/ml. (C to F) Quantitative immunofluorescence analysis of Dil-HCV internalization. Huh-7.5 cells transfected with an NT (D), AAK1 (E), or GAK (F) siRNA and expressing the early endosomal marker RAB5-GFP were infected with Dil-HCV for 1 h on ice and shifted to 37°C. Cells were fixed 1 h postinfection. Images were acquired on a DeltaVision OMX Blaze system equipped with three emCCDs. Colocalization of Dil-HCV and RAB5 was quantified by Pearson's colocalization coefficient (C). Each symbol represents an individual Dil-HCV particle; horizontal lines indicate the means ± standard deviations (error bars) (n = 121, 78, and 84 Dil-HCV particles in the NT and AAK1- and GAK-depleted cells, respectively). Data are from two independent experiments. Two representatives of Dil-HCV (red), RAB5 (green), and merged images at a magnification of ×100 are shown for each group (D to F). The small frames at the bottom correspond to boxed areas and areas with arrows. Scale bar, 10 μm. Arrows indicate Dil-HCV-RAB5 colocalization. Boxed areas indicate Dil-HCV particles that are not colocalizing with RAB5.

Article Snippet: Wide-field epifluorescence z-stacks were acquired and deconvolved on a DeltaVision OMX Blaze system (Applied Precision-GE, Inc.) equipped with three emCCDs (Evolve, Photometrics Inc.).

Techniques: Transfection, Sequencing, Infection, Virus, RNA Extraction, Quantitative RT-PCR, Control, Purification, Labeling, Reverse Transcription Polymerase Chain Reaction, Isolation, Immunofluorescence, Expressing, Marker